Gupta came from a Kabiraj family of Itna village under Lohagara police station, in the district of Jessore, presently in the Narail district of Bangladesh. He was born to Satya Ranjan Gupta and Labangalata Devi on 6 June 1911, in Kolkata where his father used to work. He spent his childhood in Kolkata. Due to his father's transferable job, he had to attend several schools, including Gaibandha High School. In 1930 he passed Matriculation from Konnagar High School. After completing his I.Sc. from Krishnanagar College, he took admission to Carmichael Medical College.^[3] While a student at the college, his elder sister died of scorpion sting. Young Gupta vowed to earn higher degree in the medical sciences to serve the ill. A part of his family also stayed at Patna, Bihar.